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Groovy script for kymograph creation

Input:

  • intensity.tiff
  • midline-binary.tiff

Analysis workflow idea:

  • for each timepoint:
  • convert binary to free line ROI object
  • set the width
  • run plot profile
  • extract the profile (coordinates and values) for each timepoint
  • find the longest profile
  • create a new image with nx = number of timepoints and ny = longest profile
  • paint values in such that we have the kymograph

Output:

  • kymograph
  • ???

Related things:

https://forum.image.sc/t/straighten-along-a-segmented-line-in-3d/41476