LocScale is a reference-based local amplitude scaling tool that used prior model information to improve contrast of cryo-EM density maps.
LocScale is a reference-based local amplitude scaling tool using prior model information to improve contrast of cryo-EM density maps. It can be used as an alternative to other commonly used map sharpening methods.
To reliably reflect the required level of local sharpening and local resolution variation in the target map, the model used for scaling should have refined B-factors using appropriate protocols. The method is described in detail in a [bioRxiv](https://doi.org/10.1101/121913) preprint [1].
To reliably reflect the required level of local sharpening and local resolution variation in the target map, atomic B-factors of the reference model should have been refined using appropriate protocols prior to scaling. The method is described in more detail in a [bioRxiv](https://doi.org/10.1101/121913) preprint [1].
