Update FA_Load_and_Correlate authored by Antonio Politi's avatar Antonio Politi
......@@ -101,19 +101,18 @@ The [**Offset**](#offset) and [**Crosstalk**](#xtalk) parameters are used for co
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<img src = './images/FA/WT_correction_idx.png' width = "400px" > | [Import](#faload) FCS measurements from WT cells into FA. The [Modify and correlate](#facorr) step is not required <br/> 1. Change tab to `Intensity corrections` <br/> 2. Check that `Autosave` is on <br/> 3. Click `Apply to` all <br/> 4. Click on `Calculate all`
<img src = './images/FA/save_res.png' width = "400px" > <br/> <img src = './images/FA/res_file_interval.png'> | 5. Change tab to `Save, export and report` <br/> 6. Click on `Save all` to save single taces <br/>7. Click on `FA format` to save a summary result table (here 2c.res)<br/> 8. Compute offset values from averages of columns named *Interval Ch1/2* in the *res* file
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<img src = './images/FA/WT_correction_idx.png' width = "400px" > | [Import](#faload) FCS measurements from WT cells into FA. The [Modify and correlate](#facorr) step is not required <br/> 1. Change tab to `Intensity corrections` <br/> 2. Check that `Autosave` is on <br/> 3. Click `Apply to` all <br/> 4. Click on `Calculate all`
<img src = './images/FA/save_res.png' width = "400px" > <br/> <img src = './images/FA/res_file_interval.png'> | 5. Change tab to `Save, export and report` <br/> 6. Click on `Save all` to save single taces <br/>7. Click on `FA format` to save a summary result table (here 2c.res)<br/> 8. Compute offset values from averages of columns named *Interval Ch1/2* in the *res* file
* <a name='xtalk'>**Crosstalk (Ch1 to Ch2)**</a>: This parameter is required when two fluorophores are measured, e.g. to quantify the cross-correlation. To quantify the crosstalk cells expressing each one of the two fluorophores are measured.
* Acquire data from cells expressing only one fluorophore (5-10 cells)
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